Normal Biometry Assessments for the Second and Third Trimester
Page Links: 1st Trimester, Gestational Age for CRL, 13 to 14 Weeks Gestation and Beyond, BPD and HC, BPD Measurements, Measurement Planes, Head Circumference (HC) Measurement, HC Measurement Plane, Differentiate the CSP from the Columns of the Fornix, Cephalic Index (CI), Dolicocephaly, Brachycephaly, Abdominal Circumference (AC) Measurement, Correct Imaging of AC, Incorrect Imaging of AC, Femoral Length (FL) Measurement, Humerus Length (HL) Measurement, Foot Measurement, Radius and Ulna Measurement, Tibia and Fibula Measurement, Orbital Measurement, Binocular Distance (BOD), Intraocular Diameter (IOD), Rate of incomplete scans during second trimester, Uterine Anomalies, References
Commentary and images courtesy of Jill Beithon RT, RDMS, RDCS, RVT
This topic covers the second and third trimester fetal age and growth assessment and will use fetal images to demonstrate the methodology and technique to acquire accurate fetal measurements.
Above. Crown rump length at 11 weeks.
The first trimester crown rump length (CRL) is the most accurate method to determine gestational age and once established should not be changed. The CRL is defined as the longest embryo length excluding the limbs and yolk sac. It is the measurements between the top of the head to the distal sacral area.
Above. Note the increased CRL with advancing gestational age.
Above. CRL versus gestational age courtesy of: “Medical Gallery of Mikael Häggström 2014,” Wikiversity Journal of Medicine.
Gestational Age for CRL
6.1 Weeks: 0.4 cm
7.2 Weeks: 1.0 cm
8.0 Weeks: 1.9 cm
9.2 Weeks: 2.5 cm
9.5 Weeks: 2.9 cm
10.5 Weeks: 4.0 cm
12.1 Weeks: 5.5 cm
13.2 Weeks: 6.90 cm
14.0 Weeks: 8.0 cm
Above. Typical gestational age compared to CRL measurement in cms. Source courtesy of: http://en.wikipedia.org/wiki/Crown-rump_length.
13 to 14 Weeks Gestation and Beyond
Beginning at 13 to 14 weeks gestation the biparietal diameter (BPD), the head circumference (HC), the abdominal circumference (AC), and femur length (FL) should be measured.
Reference values for biometry from 14 to 40 weeks can be obtained from the citation , which can be viewed at:
BPD and HC
In order to measure the BPD and HC, the transthalamic plane must be imaged. 
Above. This is the same acquisition plane for the BPD and HC. Visualization of the thalami, the midline falx, and the box-like cavum septi pellucidi (CSP) are possible. The presence or absence of the CSP is key factor in defining abnormal brain anatomy.
1. The BPD can be measured through any plane that crosses the 3rd ventricle, thalami, and cavum septi pellucidi (CSP).
2. The calvaria should be smooth and symmetrical as indicated.
3. The cursor in the near field should be placed on the outer edge of the calvaria and the cursor in the far field should be placed on the inner edge as indicated.
Head Circumference (HC) Measurement
Above. For the HC measurement:
1. Measurement is through a plane that crosses the 3rd ventricle, thalami, and CSP.
2. The calvaria should be smooth and symmetrical.
3. The cavum septi pellucidi (CSP) should be visible in the anterior portion of the brain.
4. Caliper placement is around the calvarial wall and should not include scalp thickness.
Differentiate the CSP from the Columns of the Fornix
Above. The CSP appears as a box like structure in the anterior portion of the brain as demonstrated. The hyperechoic lines represent the medial walls of the lateral ventricle.
Above. The 3-lined structure is not the CSP and represents the columns of the fornix. The fornix represents paired nerve columns, which are located just inferior to the CSP. The transaxial view when the fornix is seen suggests that the transducer plane is just inferior to the proper plane to obtain the CSP.
Above. Note the difference between the box like structure, the CSP, and the structure with 3 parallel lines which represent the columns of the fornix.
Cephalic Index (CI)
The CI is an index or ratio which is used to evaluate the shape of the head. It is calculated by measuring the maximum width (BPD) of the cranium divided by its maximum length (occipital frontal diameter, OFD). The front to back, OFD, is measured with the cursor placed on the outer edge to outer edge of the cranial bones as demonstrated. The following formula derives the cephalic index:
CI =BPD/OFD x 100
A normal cephalic index (CI) = 78.3% [6787895-refs key=”key” limit=number]
1 standard deviation (SD) = 74-83%
2 standard deviations (SD) = 70-86%
Above. Dolicocephaly is defined when the AP diameter is longer than the transverse diameter and the CI is <70% (> 2 SD). Dolicocephaly may be seen in a number of conditions, including oligohydrmnios and multiple gestations.
Above. Brachycephaly is defined when the transverse diameter is greater than the AP (anterior posterior) diameter and the CI is >86% (> 2 SD). Brachycephaly may be seen a number of conditions including synostosis, trisomy 21, and hydrocephalus.
Abdominal Circumference (AC) Measurement
Correct Imaging of AC
Above. The AC measurement should be taken at the skin line on a true transverse view at the level of the junction of the umbilical vein, portal sinus, and fetal stomach. At this location, the liver size is reflected. The visualized ribs should be symmetrical. The abdomen should not be compressed during image acquisition.
Above. Correct AC measurement. Note placement of cursor outside skin line. Again, ribs are symmetrical; the stomach is seen and both the umbilical vein and portal sinus are identified.
Incorrect Imaging of AC
Above. Incorrect imaging of AC. Note that the portal sinus is not seen, the rib images are not symmetrical, and the stomach is not well visualized.
Above. Incorrect imaging of AC. The liver is visualized but the portal sinus is not well defined; the rib images are not symmetrical and the stomach is not well visualized.
Above. Incorrect imaging of AC. The liver is visualized but the umbilical vein is not seen and the portal sinus is not well defined; the kidney should not be in the field of view. The stomach is seen but not in relationship to the umbilical vein or portal sinus.
Femoral Length (FL) Measurement
Above. The long axis of the diaphysis of the femur is most accurately measured when the ultrasound beam of insonation is perpendicular to the shaft. The cartilage at the ends of the femur should not be included in the measurement.
Above. Only the diaphysis should be measured between the epiphysis, which represents the portion of the long bone formed by the primary ossification center.
Above. Note the femoral head which is distinct from the femoral epiphysis.
Humerus Length (HL) Measurement
Above. The principles of HL measurement correspond to those of the femur. The insonation of the ultrasound beam is best directed in a perpendicular fashion and the diaphysis alone is measured after the forearm and shoulder region are identified.
The fetal foot measurement is taken from the tip of the longest toe (typically the second toe) to the tip of the heal.  A chart for fetal foot measurements and gestational age is provided at: http://www.drhern.com/pdfs/corrfetal1026.pdf
Radius and Ulna Measurement
Above. The ulna is longer than the radius, and note that it extends into the elbow, higher than the radius. The radius is on the thumb side and the ulna is on the 5th digit or little finger side of the forearm. Again, the measurement is easier to establish when the ultrasound beam is perpendicular to the long bone.
Above image is courtesy of Jane J.K. Burns, AS, RT, RDMS.
Tibia and Fibula Measurement
Among the bones of the lower leg, the fibula is smaller than the tibia. The tibia is longer than the fibula and the tibia originates nearer to the knee compared to the fibula.
Above. The orbits and the lens of the eye can be viewed when both orbits are in a coronal or axial view and most easily visualized when the fetal head is in a direct occiput posterior position.
Binocular Distance (BOD)
The BOD is measured from the outer edge of one orbit to the outer edge of the other orbit. These measurements may be useful in assessing certain syndromes, potential karyotype abnormalities, severe growth restriction, or in assessing fetal age when other measurements are difficult to obtain.
Intraocular Diameter (IOD)
The IOD represents the outer edge to outer edge distance between the orbits. It may be performed for the same reasons as BOD is measured and when facial anomalies are suspected.
Accuracy of 3-D ultrasound compared to MRI in the diagnosis of Mullerian duct abnormalities.
Three-dimensional (3-D) ultrasound compared with magnetic resonance imaging (MRI) in the diagnosis of Müllerian duct anomalies using is highly accurate.  (This article, which can be downloaded, is an excellent review of certain uterine anomalies). After being suspected of a uterine anomaly on two-dimensional scanning, 60 women were evaluated with 3D ultrasound and with MRI. Compared with MRI, 3D ultrasound had a sensitivity of 83.3% for dysmorphic uteri. For hemiuteri, the sensitivity was 100%, and the kappa was 1.00. For septate uteri, the sensitivity was 100%, and the kappa was 0.918, and for bicorporeal uteri, the sensitivity was 83.3% and the kappa was 0.900. Therefore, 3DUS is highly accurate for diagnosing uterine malformations, and has a good level of agreement with MRI in the classification of different anomaly.
Rate of incomplete scans during the second trimester
Among 4000 women who underwent a second trimester scan, a small percentage were incomplete due to unfavorable fetal position.  Among the study population, 4.2% returned for a follow-up scan within 2 weeks. Only 1 view was needed in a subsequent scan in 2.6%, while 1.6% required more than 1 view. The most difficult organs to visualize during the initial scan were: corpus callosum (1.8%), fetal face (1.7%), cerebellar vermis (1.1%), and fetal heart (1.0%).